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Yes you can, but best to do it at low volume, and be prepared for some loud pops and bangs. It shouldn't do any harm. I have wired a kill switch into my test box for just this purpose, as the noise when swapping was doing my head in.
I tend to line up my alternative candidates next to the pedal, orientated the right way round for the sockets. I stick the legs into a strip of Blu tac on a piece of card, with the names of the transistors written above them. This makes the process easier.
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